With the acquisition of Intelligent Biosystems in 2012, Qiagen became a sequencing platform company. So far they haven’t shared too many details on their upcoming platform, the GeneReader, other than it appears to be based on the IBS Mini-20. Originally slated to launch in late 2012, they are now targeting a mid-2014 launch. Like the […]
The latest developments in the next ‘next generation’ sequencing platforms that are promising to dethrone the current market entrants.
General Overview QuantuMDx, while still in ‘semi-stealth mode’, is developing handheld sequencing instruments based on microfluidic channels and nanowires. Q-POC The first instrument they will likely launch is the Q-POC, a handheld clinical short read sequencer. The system will contain a series of nanowires to which template DNA is attached. DNA bases are then […]
Reveo is creating a next-gen sequencer based on atomic force microscopy via their ReVase subsidiary. The system, called the Omni Molecular Recognizer Application (OmniMoRA), will use arrays of nano-knife edge probes to measure the vibrational characteristics of individual bases on DNA molecules that have been stretched and immobilized on a surface. While Reveo has not […]
Stratos Genomics is building a nanopore-based single molecule sequencer. However, rather than directly sequencing the native DNA molecule, they have developed a process they call ‘sequencing-by-expansion’. They convert the DNA into an expanded molecule, or ‘xpandomer’, through a process similar to replication via polymerase. However, rather than incorporating a single base at a time into […]
Two Pore Guys is an early stage technology development company that is attempting to address the issue of DNA flow rate through nanopores. Instead of reading strands of DNA through a single pore, they are using two pores in series such that the DNA molecule is held by both. By applying opposing currents through the […]
ZS Genetics is developing a single molecule sequencer based on annular dark-field scanning transmission electron microscopy (ADF-STEM). Since native DNA is invisible to the STEM imager, they’ll be incorporating ‘ZSG labels’, modified nucleotides with heavy atoms or other compounds to make them visible. The modified nucleotides will be incorporated via a template-directed DNA polymerase (essentially […]