454 was the first of the NGS platforms on the market, launching in 2005. It was acquired by Roche in 2007 who, in October 2013, announced that they were shutting down the business by mid-2016. Genome Sequencer FLX+ The GS FLX System is 454′s flagship sequencing system. It uses emulsion PCR (emPCR) to clonally amplify […]
All the key info about the sequencing platforms that are currently available on the market gathered together, including platform specs and platform-application suitability.
General Overview Complete Genomics is unique in the NGS market in that instead of selling their proprietary instrumentation they use it to offer NGS services. They have chosen to focus on a single application: human whole-genome resequencing. At the moment they don’t offer any other applications (e.g., exome, RNA-Seq, ChIP-Seq, etc). Because they operate solely […]
General Overview Illumina officially entered the next-gen sequencing market with their acquisition of Solexa in late 2006. Despite not being the first to market, this platform has become the dominant next-gen sequencing technology. The workflow involves the use of bridge amplification to clonally amplify the fragments that are then sequenced using sequencing-by-synthesis (SBS) chemistry. Illumina […]
General Overview Life Technologies (now ThermoFisher) expanded their NGS portfolio with the acquisition of Ion Torrent and their first system, the Ion Personal Genome Machine (PGM) sequencer, in 2010. While this technology uses a sequencing-by-synthesis method and emulsion PCR (emPCR) similar to other platforms, it differs in that it doesn’t use fluorescence or chemiluminescence. Instead, […]
Oxford Nanopore Technologies (ONT) is hoping to develop the first commercially available nanopore-based DNA sequencer. They have yet to show any actual DNA sequencing data, but they do have a very polished looking platform. Their GridION system currently uses protein (alpha-hemolysin) nanopores but they are also working on solid state nanopores. They are developing two […]
Overview Pacific Biosciences (often abbreviated PacBio; previously named Nanofluidics), rather than taking the NGS field head on, has carved out a niche by focusing on how they can complement and supplement other sequencing platforms. Rather than generating a large number of relatively short reads, PacBio generates a relatively small number of very long reads. These […]
Life Technologies’ (now ThermoFisher) SOLiD sequencing platform uses emulsion PCR (emPCR) and a sequencing-by-ligation approach that generates results in “color space.” 5500xl SOLiD The 5500xl SOLiD system is Life Technologies’ flagship sequencer. The official website specifications are listed at up to 180 Gb per run, 2 x 60 bp reads, up to 20-30 Gb per […]