General Overview Illumina officially entered the next-gen sequencing market with their acquisition of Solexa in late 2006. Despite not being the first to market, this platform has become the dominant next-gen sequencing technology. The workflow involves the use of bridge amplification to clonally amplify the fragments that are then sequenced using sequencing-by-synthesis (SBS) chemistry. Illumina […]
All the key info about the sequencing platforms that are currently available on the market gathered together, including platform specs and platform-application suitability.
General Overview Life Technologies (now ThermoFisher) expanded their NGS portfolio with the acquisition of Ion Torrent and their first system, the Ion Personal Genome Machine (PGM) sequencer, in 2010. While this technology uses a sequencing-by-synthesis method and emulsion PCR (emPCR) similar to other platforms, it differs in that it doesn’t use fluorescence or chemiluminescence. Instead, […]
Overview Oxford Nanopore is the first company to commercially launch a nanopore based DNA sequencer. Nanopores are small holes through which DNA can travel, generating an electronic signal that is used to sequence the individual bases. Oxford Nanopore sequencers, which are inexpensive to own and operate, are able to generate very long reads, albeit with […]
Overview Pacific Biosciences (often abbreviated PacBio; previously named Nanofluidics), rather than taking the NGS field head on, has carved out a niche by focusing on how they can complement and supplement other sequencing platforms. Rather than generating a large number of relatively short reads, PacBio generates a relatively small number of very long reads. These […]
With the acquisition of Intelligent Biosystems in 2012, Qiagen became a sequencing platform company. Their upcoming platform, the GeneReader, is based on the IBS Mini-20. Originally slated to launch in late 2012, the GeneReader didn’t reach the market until November 2015. Like the original, it is based on multiple independently addressable flow cells, each presumably capable […]